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Construct size (kb): 6.800000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmLR, tetR
replicon: pMB1
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MKI67
Gene name: antigen identified by monoclonal antibody Ki-67
Contains complete coding sequence?: N
Chromosome: 10; Localization: 10 q25-qter
Tissue: IM-9 cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.092
Cross references: DNA Seq. Acc.: X65550

Insert lengths(kb): 1.092000007629395

Tissue: IM-9 cell line

Gene product: antigen identified by monoclonal antibody Ki-67( Proliferation-related Ki-67 antigen, Ki-67 antigen) [MKI67]

Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 1.1; HindIII--7.0; PstI--5.0, 2.1; SacI--7.1; XbaI--7.1.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--1.00; SacI--0.21, 0.57, 0.73, 0.94; XbaI--0.18.

It includes three of sixteen Ki-67 repeats.

Detects splicing isoforms of 8.7 and 9.8 kb.