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pACYC184
pACYC184
規(guī)格:
貨期:
編號(hào):B211873
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pACYC184
商品貨號(hào) B211873
Designations pACYC184
Depositors SN Cohen
Biosafety Level 1
Host Escherichia coli PM191
Vector Information Vector size (kb): 4.244
Markers: cmlR, tetR
DNA Seq. Acc.: X06403

Type of vector: plasmid
Cloning sites: EcoRI HindIII BamHI SalI
Construction: p15A, pSC101, Tn9
Host range: Escherichia coli
Features):
marker(s): cmlR, tetR
replicon: p15A

Suggested host(s):
Escherichia coli chi-1776 (ATCC® 31244™)
Escherichia coli chi-1776 (ATCC® 31537™)
Escherichia coli chi-1776 (ATCC® 39234™)
Escherichia coli chi-1776 (ATCC® 53705™)
Escherichia coli C600 (ATCC® 23724™)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9.
Low copy number plasmid vector containing the replication system of miniplasmid p15A.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37°C
References

Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110

Rose RE. The nucleotide sequence of pACYC184. Nucleic Acids Res. 16: 355, 1988. PubMed: 3340533

Shipped frozen
Shipping Information Frozen glycerol stock of Escherichia coli containing the plasmid.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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