亚洲精品无码成人片久久不卡,亚洲自偷自偷在线传媒,亚洲精品在线,久久无码精品九号,欧洲av人人爽爽,亚洲精品蜜桃久久久久久,亚洲精品久久午夜无码专区电影,辽宁少妇高潮45分钟,少妇高清性色生活片,无码八少妇久久

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > Trypanosoma cruzi Chagas
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Trypanosoma cruzi Chagas
Trypanosoma cruzi Chagas
規(guī)格:
貨期:
編號(hào):B210262
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Trypanosoma cruzi Chagas
商品貨號(hào) B210262
Strain Designations M/HOM/BR/78/SYLVIO-X10 CL4
Application
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Clone 4 derived from strain SYLVIO-X10 (= ATCC® 50823™)
Product Format frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain no
Comments
Flow cytometric analysis
Medium ATCC® Medium 1029: LIT medium
Growth Conditions
Temperature: 25°C
Culture System: Axenic
Cryopreservation Harvest and Preservation
  1. Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).  The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.  Do not agitate the ampule.  Do not leave ampule in water bath after thawed.
  9. Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh flask containing 10 mL ATCC medium 1029. 
  10. Incubate at 25°C with the cap screwed on tightly.
  11. Maintain as described above. 
Name of Depositor JA Dvorak
Special Collection NCRR Contract
Chain of Custody
ATCC <-- JA Dvorak <-- M.A. Miles <-- F.T. Silveira
References

Dvorak JA, et al. Trypanosoma cruzi: flow cytometric analysis. I. Analysis of total DNA/organism by means of mithramycin-induced fluorescence. J. Protozool. 29: 430-437, 1982. PubMed: 6182288

Miyahira Y, Dvorak JA. Kinetoplastidae display naturally occurring ancillary DNA-containing structures. Mol. Biochem. Parasitol. 65: 339-349, 1994. PubMed: 7969274

Scott DA, et al. In situ compositional analysis of acidocalcisomes in Trypanosoma cruzi. J. Biol. Chem. 272: 28020-28029, 1997. PubMed: 9346954

Postan M, et al. Studies of Trypanosoma cruzi clones in inbred mice. III. Histopathological and electrocardiographical responses to chronic infection. Am. J. Trop. Med. Hyg. 37: 541-549, 1987. PubMed: 3318521

Nozaki T, Dvorak JA. Trypanosoma cruzi: flow cytometric analysis of developmental stage differences in DNA. J. Protozool. 38: 234-243, 1991. PubMed: 1880761

Postan M, et al. Studies of Trypanosoma cruzi clones in inbred mice. I. A comparison of the course of infection of C3H/HEN- mice with two clones isolated from a common source. Am. J. Trop. Med. Hyg. 32: 497-506, 1983. PubMed: 6407346

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
石景山区| 乐东| 南宁市| 措勤县| 南宫市| 新巴尔虎左旗| 志丹县| 定远县| 仁化县| 晴隆县| 青浦区| 东源县| 呼伦贝尔市| 黄浦区| 美姑县| 金沙县| 本溪| 昌黎县| 六安市| 霞浦县| 永昌县| 广安市| 嘉善县| 洪江市| 简阳市| 涞水县| 比如县| 武功县| 青海省| 和田县| 民丰县| 南阳市| 黎川县| 武威市| 新营市| 湖口县| 庐江县| 沅陵县| 宝鸡市| 广宁县| 仙游县|