| 產(chǎn)品名稱(chēng) |
T-VCAM-1 [clone L-59] |
| 商品貨號(hào) |
B209554 |
| Designations |
T-VCAM-1 [clone L-59] |
| GenBank Number |
U12884
|
| Species |
Mus musculus, mouse |
| Depositors |
CM Ballantyne |
| Applications |
contains sequence vascular cell adhesion molecule-1 vascular cell adhesion molecule 1 |
| Vector |
Construct size (kb): 4.400000095367432 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.399999976158142 Tissue: liver, LPS-treated mouse Gene product: vascular cell adhesion molecule-1(vascular cell adhesion molecule 1) [Vcam1] Target Gene: vascular cell adhesion molecule-1 |
| Insert Size (kb) |
1.400 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): NdeI--4.3; SphI--4.4; SacI/XhoI--2.9, 1.4. Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.35; BglII--0.08; HindIII--0.07; NcoI--0.12, 0.24; NdeI--1.00; PvuI--0.10; SphI--1.20; XbaI--0.52. Encodes a truncated form of Vcam1 including Ig domains 1-3 and having a different carboxy terminus. The 5' end of the gene was generated by PCR and was ligated to the 3' portion of the clone N10 at the BamHI site. The resulting full length cDNA was confirmed by sequencing. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Kumar AG, et al. Murine VCAM-1. Molecular cloning, mapping, and analysis of a truncated form. J. Immunol. 153: 4088-4098, 1994. PubMed: 7523515
|
