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Size (kb): 6.0770001411438000

Vector: pZilch (phagemid)

Promoters: Promoter CMV

Construction: pCATCH

Marker(s):ampR

Construct size (kb): 6.0770001411438

Features: marker(s): ampR
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: BamHI...XhoI
MCS: BglII...SalI
terminator: SV40
coding sequence: mutant pheS gene
epitope tag: FLAG

encodes removable tag for protein isolation

expression vector

vector permitting construction of fusion proteins

vector permitting positive selection for inserts

vector permitting production of single-stranded DNA

Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1.

The vector was contructed by inserting a 1185 bp SmaI/BamHI fragment from pKSS encompassing the mutant pheS allele into the pCATCH and modified further to generate polylinker and to incorporate Kozak sequence and initiation ATG codon.

Temperature: 37.0°C