| 產(chǎn)品名稱 |
HHCMH42 |
| 商品貨號(hào) |
B208750 |
| Designations |
HHCMH42 |
| GenBank Number |
M78047
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 4.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D0S1050E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene symbol: TAX
Other gene symbols: TAG1
Genomic copy number: unique
Gene name: transiently-expressed axonal glycoprotein (axonin-1)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 q32
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.9
Cross references: DNA Seq. Acc.: M78047; EST: EST01634; Primer: M13 Reverse;
THC id: THC109655
DNA Seq. Acc.: X68274
Nucleotides 1-237 of the insert correspond to
nucleotides 2690-2925 of X68274 with 97% identity. Insert lengths(kb): 1.899999976158142 Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205) Gene product: axonal glycoprotein TAG-1( axonal glycoprotein TAG-1, axonin-1 cell adhesion molecule, transiently-expressed axonal glycoprotein, transiently-expressed axonal glycoprotein (axonin-1)) [TAX] |
| Insert Size (kb) |
1.900 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.9; PstI--3.1, 1.1, 0.34, 0.21; BamHI--4.2, 0.50. EST01634 matches PIR accession A34695 with 87.14% identity and 94.28% similarity over the sequence range: 1-210. Sequenced from the M13 reverse primer. This sequence record is for rat axonal glycoprotein TAG-1. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Hasler TH, et al. cDNA cloning, structural features, and eukaryotic expression of human TAG-1/axonin-1. Eur. J. Biochem. 211: 329-339, 1993. PubMed: 8425542
J C Venter, personal communication
|
