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產(chǎn)品名稱 pTB10.163
商品貨號 B208406
Designations pTB10.163
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
RFLPs were observed under normal hybridization and wash stringencies.
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: genomic
Insert lengths(kb): 2.700000047683716
Gene product: DNA Segment, single copy [D10S22]
Alleles: A3, A1, A2
Insert Size (kb) 2.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.6; HindIII--5.6; EcoRI/HindIII--2.8, 2.8.
Codominant segregation demonstrated in 47 3-generation families for the MspI RFLP.
RFLPs were observed under normal hybridization and wash stringencies.
Enzyme(s) not detecting polymorphism: TaqI, RsaI, PstI, BglII, PvuII.
References

Wu JS, et al. The genetic defect in multiple endocrine neoplasia type 2A maps next to the centromere of chromosome 10. Am. J. Hum. Genet. 46: 624-630, 1990. PubMed: 1968709

White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325

Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050

Bragg T, et al. Isolation and mapping of a polymorphic DNA sequence (pTB10.163) on chromosome 10 [D10S22]. Nucleic Acids Res. 16: 4185, 1988. PubMed: 2453849

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