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pUCM11
pUCM11
規(guī)格:
貨期:
編號:B242836
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUCM11
商品貨號 B242836
Designations pUCM11
GenBank Number

M14211

Species Homo sapiens, human
Applications
produces protein ferritin, heavy polypeptide 1 Ferritin subunit H
Vector
Construct size (kb): 9.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 7.199999809265137
Tissue: liver
Gene product: ferritin, heavy polypeptide 1(Ferritin subunit H) [FTH1]
Target Gene: ferritin, heavy polypeptide 1
Insert Size (kb) 7.200
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--7.0, 2.7; BamHI--4.3, 2.8, 2.5; EcoRI--6.6, 3.3; PstI--4.0, 2.8, 1.3, 0.8, 0.7, 0.5; SalI--9.8.
The insert contains the entire coding sequence (4 exons) plus upstream and downstream sequences. The EcoRI fragment in the insert is smaller than the genomic sequence it detects, suggesting truncation during construction.
Only a partial sequence of promoter, intron I, all exons and introns II and III is available.
Insert is expressed in transformed murine B6 fibroblasts.
A 0.7 kb PstI fragment which is upstream of the TATA sequence is unique and was used to map the gene. It does not cross-hybridize with hamster or mouse DNA.
References

Hentze MW, et al. Cloning, characterization, expression, and chromosomal localization of a human ferritin heavy-chain gene. Proc. Natl. Acad. Sci. USA 83: 7226-7230, 1986. PubMed: 3020541

Hentze MW, et al. A cis-acting element is necessary and sufficient for translational regulation of human ferritin expression in response to iron. Proc. Natl. Acad. Sci. USA 84: 6730-6734, 1987. PubMed: 3477805

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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