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pMNS01A
pMNS01A
規(guī)格:
貨期:
編號:B232678
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pMNS01A
商品貨號 B232678
Designations pMNS01A
GenBank Number

D12536

M29671

Species Melon necrotic spot virus, Melon necrotic spot virus
Depositors DM Rochon
Applications
contains sequence genome, partial, 3' end
Vector
Construct size (kb): 4.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 2.150000095367432
Gene product: genome, partial, 3' end
Target Gene: genome, partial, 3' end
Insert Size (kb) 2.150
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.6, 2.4 (partial); HindIII--3.4, 1.5; BamHI--3.2, 1.35, 0.35.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.2, 1.55; BglII--1.0; ClaI--1.7; EcoRI--0.1; EcoRV--1.95; HindIII--0.75; KpnI--1.8; NcoI--0.3, 1.45; SalI--1.4; SmaI--0.6, 1.6.
Virus was isolated from cucumber, Cucumis sativus.
The insert hybridizes to the genomic length RNA (4.26 kb) and two shorter RNAs of about 1.9 kb and 1.6 kb.
Classification Carmovirus
References

Riviere CJ, Rochon DM. Nucleotide sequence and genomic organization of melon necrotic spot virus. J. Gen. Virol. 71: 1887-1896, 1990. PubMed: 2212985

D'Ann M Rochon, personal communication

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