| 產(chǎn)品名稱 |
alphaRI12 |
| 商品貨號(hào) |
B216873 |
| Designations |
alphaRI12 |
| Species |
Homo sapiens, human |
| Depositors |
YB Yurov |
| Vector |
Construct size (kb): 6.45
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR, tetR
replicon: pMB1 |
| Insert |
Genome: Homo sapiens
Gene symbol: D6Z1
Genomic copy number: repetitive
Gene name: DNA Segment, repetitive
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 cen
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 0.34
Genome: Homo sapiens
Gene symbol: D6Z1
Type of nucleic acid: genomic
Restriction enzyme: TaqI
Restriction enzyme: BamHI |
| Insert Size (kb) |
0.340 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--6.3, 0.3; PstI--6.6; SalI--6.7; BglI--2.6, 2.4, 1.2 + smaller; PvuII--3.8, 2.8. Similar to p308. |
| References |
Yurov YB, et al. Collection of alpha-satellite DNA probes: highly polymorphic markers for centromeric regions of all human chromosomes. Cytogenet. Cell Genet. 51: 1114, 1989.
Yu B Yurov, personal communication
|
